Analytical method for the determination of aromatic amines
Translation of DGUV Information 213-583 (former BGI 505-83 or ZH1/120.83)
| Chemical name | CAS Number |
|---|---|
| Aniline; aminobenzene | 62-53-3 |
| 2,4-Dimethylaniline; 2,4-xylidine | 95-68-1 |
| 2,5-Dimethylaniline; 2,5-xylidine | 95-78-3 |
| 2,6-Dimethylaniline; 2,6-xylidine | 87-62-7 |
| 4-Ethoxyaniline; p-phenetidine | 156-43-4 |
| 2-Methoxyaniline; o-anisidine | 90-04-0 |
| 3-Methoxyaniline; m-anisidine | 536-90-3 |
| 4-Methoxyaniline; p-anisidine | 104-94-9 |
| 2-Methylaniline; o-toluidine | 95-53-4 |
| 3-Methylaniline; m-toluidine | 108-44-1 |
| 4-Methlylaniline; p-toluidine | 106-49-0 |
Method 02 – GC
Sampling is carried out with a pump and adsorption on a filter impregnated with acid. Analysis is performed by gas chromatography after desorption.
This analytical method is a validated measurement procedure for the determination of 2-methylaniline, 3-methylaniline, 4-methylaniline, 1-naphthylamine, 2-naphthylamine, 2-aminobiphenyl, 3-aminobiphenyl and 4-aminobiphenyl in workplace air averaged over the sampling period after personal or stationary sampling.
Principle
A pump draws a defined volume of air through a cassette equipped with two acid-impregnated filters. The deposited amines are then transferred to an aqueous solution, extracted with toluene in an alkaline medium and derivatized with heptafluorobutyric anhydride. After gas chromatographic separation, determination is carried out with the mass selective detector.
Limit of quantification (LOQ)
Absolute: 0.04 to 0.23 pg per amine.
Relative: 0.001 to 0.008 μg/m³ of amine for an air sample volume of 120 l, a desorption solution of 2 ml and an injection volume of 0.5 μl.
Issue
May 2020
The complete measuring method is available in the ZB MED: Method for the determination of aromatic amines in workplace air using gaschromatography (The MAK Collection for Occupational Health and Safety, Volume 5, No. 3, 2020).
Method 01 – GC
Sampling is carried out with a pump and adsorption on a filter impregnated with acid. Analysis is performed by gas chromatography after desorption.
The method permits the determination of aniline, 4-ethoxyaniline, methoxyanilines, methylanilines and dimethylanilines concentrations – abbreviated to amines in this method – in working areas averaged over the sampling time after personal or stationary sampling.
Principle
A defined volume of air is drawn through a filter holder equipped with a filter impregnated with acid using a pump. The adsorbed amines are then desorbed from the filter with a mixture of acetonitrile/ammonia solution and determined by a mass selective detector (MSD) after separation by means of gas chromatography.
Limit of quantification (LOQ)
Absolute: 0.2 to 1.6 ng per amine.
Relative: 0.003 to 0.026 mg/m3 of an amine for an air sample volume of 240 L, 4 mL of desorption solution and an injection volume of 1 µL.
Issue
June 2012
The complete measuring method is available in the Wiley Online Library: Method for the determination of aromatic amines (Air Monitoring Methods, 2015).
You can reach the German-language version of the article with a single click on the flag-symbol.
