Analytical method for the determination of acrylamide
Translation of DGUV Information 213-537 (former BGI 505-37 or ZH1/120.37)
CAS Number: 79-06-1
Synonyms:
- 2-Propenamide
- Acrylic acid amide
Method 04 – GC/MS – limited suitability: category E3
Sampling is carried out by means of a pump and collection on a charcoal filter. Analysis is performed by gas chromatography with mass selective detector (GC/MS) after elution.
This method permits the determination of acrylamide concentrations in work areas averaged over the sampling time after personal und stationary sampling.
Principle
A pump is used to draw a measured volume of air through a GSP-sampling system equipped with a charcoal filter. The inhalable dust fraction of acrylamide is collected on the GSP-sampling system according to EN 481, whereas gaseous acrylamide is adsorbed on a charcoal filter. The collected acrylamide is eluted with a mixture of dichloromethane and methanol (9 + 1 v/v) and determined by gas chromatography with mass selective detector.
Limit of quantification (LOQ)
Absolute: 0.1 ng acrylamide.
Relative: 1 µg/m³ acrylamide for a 210-litre air sample, 2 mL elution solution and an injection volume of 2 µL.
Issue
December 2004
The measuring method is of limited suitability (E3).
The complete measuring method is available in the Wiley Online Library: Method for the determination of acrylamide (Air Monitoring Methods, Volume 11, 2009).
Method 03 – GC – withdrawn
Sampling with a pump and collection of aerosols on a glass-fibre filter with subsequent adsorption von activated carbon. Gas chromatography after desorption.
This method permits the determination of acrylamide concentrations in working areas averaged over the sampling time after personal und stationary sampling.
Principle
With a pump a measured air volume is drawn through a sampling system. The system contains a glass-fibre filter for collecting the inhalable dust fraction in accordance to DIN EN 481 and behind it activated carbon to adsorb the vapour. The acrylamide collected is desorbed with a mixture of dichloromethane/methanol and determined by gas chromatography.
Limit of quantification (LOQ)
Absolute: 0.8 ng acrylamide.
Relative: 0.006 mg/m³ acrylamide for a 420-litre air sample, 3 ml elution solution and 1 µl injection volume.
Issue
October 1990
Withdrawn
February 2016
The complete measuring method is available in the Wiley Online Library: Method for the determination of acrylamide (Air Monitoring Methods, Volume 7, 2003).
Method 02 – HPLC – withdrawn
Sampling with a pump and adsorption on silica gel. High-performance liquid chromatography (HPLC) after desorption.
This method permits the determination of acrylamide concentrations in working areas averaged over the sampling time after personal und stationary sampling.
Principle
With a pump a measured air volume is drawn through a tube filled with silica gel. The adsorbed acrylamide is then desorbed with phosphoric acid and determined by high-performance liquid chromatography.
Limit of quantification (LOQ)
Absolute: 6 ng acrylamide.
Relative: 0.003 mg/m³ acrylamide for a 100-litre air sample, 10 ml sample solution and 200 µl injection volume.
Issue
August 1987
Withdrawn
May 2014
The complete measuring method is available in the Wiley Online Library: Method for the determination of acrylamide (Air Monitoring Methods, Volume 7, 2003).
Method 01 – GC – withdrawn
Sampling with a pump and adsorption on activated carbon. Gas chromatography after desorption.
This method permits the determination of acrylamide concentrations in work areas averaged over the sampling time after personal und stationary sampling.
Principle
With a pump a measured air volume is drawn through a tube filled with activated carbon. The adsorbed acrylamide is then desorbed with a mixture of dichloromethane/methanol and determined by gas chromatography.
Detection limit
Absolute: 1.5 ng acrylamide.
Relative: 0.003 mg/m³ acrylamide for a 500-litre air sample.
Issue
August 1987
Withdrawn
December 2004
The complete measuring method is available in the Wiley Online Library: Method for the determination of acrylamide (Air Monitoring Methods, Volume 7, 2003).
You can reach the German-language version of the article with a single click on the flag-symbol.
